CHARACTERIZATION OF LACTATE DEHYDROGENASE ISOZYME PATTERN AND MORPHOLOGY OF THREE MARINE FISH CELL LINES     

郭华荣  张士璀  李红岩  童裳亮  相建海 《中国海洋湖沼学报》2002,20(3):226-231

Three continuous marine fish cell lines of FG (i. e., Hounder Gill) from flounder (Paralichthys olivaceus) gill, SPH (i. e. , Sea Perch Heart) from sea perch (Lateolabrax japonicus) heart and RSBF (i.e., Red Sea Bream Fin) from red sea bream (Pagrosomus major) fin, were characterized by lactate dehydrogenase (LDH) isozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results showed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However, the LDH isozyme patterns of SPH and RSBF were significantly different from their corresponding tissues of origin; (2) LDH isozyme patterns of FG, SPH and RSBF were markedly different from each other and could serve as genetic markers for species identification and detection of cross contamination. Morphological change analysis of these three cell lines in comparison to their original tissues indicated that FG cells still appeared epithelioid without morphological transformation. However, morphological changes were found in SPH and RSBF compared to their original tissues. Therefore, the cellular morphology was still plastic in the relatively stable culture conditions, and it was possible that change of LDH patterns wasrelated to morphological changes of fish cells in vitro.  相似文献   

天文光干涉与综合孔径技术的发展     

郭红锋 《紫金山天文台台刊》2003,(1)

本文简单介绍了光干涉与综合孔径技术发展的历史、国际上用于天文观测的一些著名光干涉仪的技术特点和进展、以及近年来我国开展的与光干涉 ,综合孔径技术有关的工作 ,最后简要介绍了在光学综合孔径技术领域所发展的光纤与集成光学等新技术  相似文献   

丽江高美古台址和2m级望远镜项目(英文)     

陈东  王建成  许骏  罗国权  谭徽松  刘忠  张柏荣 《天文研究与技术》2003,(Z1)

自 1992年起云南天文台开始了在云南省西北部的天文选址。丽江高美古最终被选择作为未来中国地基天文光学观测的新址点。并且 ,在国家科技部 ,云南省政府 ,以及中科院的支持下 ,2m级天文望远镜项目在 2 0 0 0年 12月获得立项。在文中 ,我们简要的介绍了高美古台址的情况 ,并给出了 2 .4m望远镜的详细情况 ,以及其他相关情况。  相似文献   

EFFECT OF SULPHATE-REDUCING BACTERIA ON ELECTRO-CHEMICAL CORROSION BEHAVIOR OF 16Mn STEEL IN SEA MUD     

张经磊  侯保荣  郭公玉  孙虎元  徐岩 《中国海洋湖沼学报》2001,19(1):87-90

ＩＮＴＲＯＤＵＣＴＩＯＮＩｔｗａｓｇｅｎｅｒａｌｌｙｃｏｎｓｉｄｅｒｅｄｐｒｅｖｉｏｕｓｌｙｔｈａｔｔｈｅｃｏｒｒｏｓｉｏｎｏｆｓｔｅｅｌｉｎｓｅａｍｕｄｗａｓｎｏｔｓｅｒｉｏｕｓ.Ｈｏｗｅｖｅｒ,ｉｔｗａｓｌａｔｅｒｆｏｕｎｄｔｈａｔｔｈｅｃｏｒｒｏｓｉｏｎｒａｔｅｏｆｓｔｅｅｌｉｎｓｏｍｅｓｅａｍｕｄｗａｓｈｉｇｈｅｒｔｈａｎｔｈａｔｉｎｓｅａｗａｔｅｒ(Ｋｉｎｇ,1980;Ｓｅｈｍｉｄｅ,1982;Ｇｕｏｅｔａｌ.,1997;Ｈｏｕ,1998).Ｔｈｅｒｅａｒｅｓｏｍｅｒｅｐｏｒｔｓｔｈａｔｔｈｅｃｏｒｒｏｓｉｏｎｒ…  相似文献   

Automated Separation of Stars and Normal Galaxies Based on Statistical Mixture Modeling with RBF Neural Networks     

Dong-Mei Qin  Ping Guo  Zhan-Yi Hu  Yong-Heng ZhaoNational Laboratory of Pattern Recognition Laboratory  Institute of Automation  Chinese Academy of Sciences  Beijing  dmqin@nlpr.ia.ac.cnDepartment of Computer Sciences  Beijing Normal University  Beijing National Astronomical Observatories  Chinese Academy of Sciences  Beijing 《中国天文和天体物理学报》2003,3(3)

For LAMOST, the largest sky survey program in China, the solution of the problem of automatic discrimination of stars from galaxies by spectra has shown that the results of the PSF test can be significantly refined. However, the problem is made worse when the redshifts of galaxies are not available. We present a new automatic method of star/(normal) galaxy separation, which is based on Statistical Mixture Modeling with Radial Basis Function Neural Networks (SMM-RBFNN). This work is a continuation of our previous one, where active and non-active celestial objects were successfully segregated. By combining the method in this paper and the previous one, stars can now be effectively separated from galaxies and AGNs by their spectra-a major goal of LAMOST, and an indispensable step in any automatic spectrum classification system. In our work, the training set includes standard stellar spectra from Jacoby's spectrum library and simulated galaxy spectra of EO, SO, Sa, Sb types with redshift ranging from 0 to 1  相似文献   

The Dependence of Water Exchanges between the Kuroshio Water and Its Surroundings on the Characteristics of the Kuroshio‘s Meanders     

姜华  吴德星  万修全 《中国海洋湖沼学报》2003,21(4):335-344

Observation of fluid parcel pathways in the Kuroshio Stream revealed a striking crossstream pattern associated with the Kuroshio‘‘ s meanders. In order to understand the observed pattern, a two-dimensional kinematic model of a meandering jet was developed which could be used to examine the relationship between streamfunction patterns and fluid parcel trajectories. The experiments designed to investigate the dependence of the water exchanges between the Kuroshio water and its surroundings on the characteristics of the Kuroshio‘‘s meanders involved the downstream speed,phase speed and the amplitude of other propagating waves. The results suggested that fluid parcels could cross the meandering jet to and fro; and that the water exchanges between the Kuroshio and its surroundings increased with a) increasing meander amplitude, b) decreasing downstream speed, and c) increasing wave phase speed. The results also showed that when the meandering jet was disturbed by other propagating waves, more effective water exchanges could be induced.  相似文献   

临沂市土地开发整理成效显著          下载免费PDF全文

郭兴雷 《山东国土资源》2006,22(5):12-12,14

2005年临沂市国土资源局在山东省国土资源厅和临沂市委、市政府的正确领导下，始终把土地开发整理作为实现全市耕地占补平衡，保障经济社会可持续发展和服务“三农”，增加农民收入的致富工程、民心工程、德政工程来抓，取得了明显成效。  相似文献   

高能偏振能量色散X射线荧光光谱仪应用现状和进展   总被引：4，自引：2，他引：2  

吉昂  李国会  张华 《岩矿测试》2008,27(6):451-462

对高能偏振能量色散X射线荧光光谱仪基本特点及其用于环境、生物、地质样品中痕量元素的分析和RoSH检测等领域的应用现状予以介绍,并对二次靶与滤光片在痕量元素分析中的应用作了探讨。  相似文献   

火试金法测定铜精矿中金含量结果的不确定度评定   总被引：2，自引：0，他引：2  

江水英  肖化云  吴声东  吴波  秦云和 《岩矿测试》2008,27(4):299-301

对火试金法测定铜精矿中金含量的结果进行不确定度评定。分析了铜精矿样品称量、铜精矿样品的不均匀性和配料处理,以及金粒称量等因素对金含量测量结果不确定度的影响,并得出火试金法测量铜精矿中金含量的扩展不确定度。  相似文献   

Plant distribution at the mobile dune scale and its relevance to soil properties and topographic features     

Xiaoan Zuo  Halin Zhao  Xueyong Zhao  Yirui Guo  Yulin Li  Yayong Luo 《Environmental Geology》2008,54(5):1111-1120

A survey was conducted in an 11-year recovery mobile dune (RMD11) and a 20-year recovery mobile dune (RMD20), in Horqin Sandy Land, Northern China, to determine plant distribution at the mobile dune scale and its relevance to soil properties and topographic features. The results showed that (1) vegetation cover and species number increased from dune top to bottom in the restoration process of mobile dune; (2) the average value of soil organic C, total N, pH, relative height of sampling site, very fine sand content and soil water contents (40−60 and 60−80 cm) of RMD11 were less than that of RMD20, respectively, and there were significant differences (P < 0.05) between the two dunes; (3) soil resources were redistributed by shrub restoration and relative height of sampling site on dune. The distribution of sand pioneer plant, Agriophyllum squarrosum, was positively related to the relative height of sampling site and soil water content, while that of other herbaceous plants was positively related to soil nutrients in the restoration process of mobile dune. These results suggest that at mobile dune scale, plant distributions are determined by a combination of soil properties and topographic feature. Much effort should be made to preserve the interdune lowland and to improve the level of soil nutrients on mobile dune.  相似文献